MCAT Question of the Day
    •

    Q:

    		 A plasmid contains genes for ampicillin and kanamycin resistance. A restriction endonuclease is used to cut the plasmid within the ampR gene before ligating in a gene fragment, that shares the same restriction sites cut by the endonuclease, encoding green fluorescent protein (GFP). The product from this plasmid construct experiment is then transformed into competent bacteria, which are allowed to grow and replicate over a period of time. These bacteria are then smeared on agar plates containing kanamycin. The next day, several bacterial colonies are observed to grow on the plates. Viewed under a fluorescent microscope, fluorescence is observed. Which of the following is true about all of the bacterial colonies that are growing on the agar plates?
    		 

    A

    		 They all contain the plasmid construct containing GFP.

    B

    		 They all exhibit ampicillin resistance.

    C

    		 They all have kanamycin antibiotic resistance

    D

    		 None of the above is true.



    Recombinant DNA and Biotechnology |
    Subscribe


    Connect

    Questions? We're here to help!

    Ask Us